ABSTRACT
A cytoplasmic male sterile (CMS) line of Brassica juncea was derived by repeated backcrossing of the somatic hybrid (Diplotaxis catholica + B. juncea) to B. juncea. The new CMS line is comparable to euplasmic lines for almost all characters, except for flowers which bear slender, needle-like anthers with aborted pollen. Detailed Southern analysis revealed two copies of coxI gene in the CMS line. One copy, coxI-1 is similar to the coxI gene of B. juncea, whereas the second copy, coxI-2 is present in a novel rearranged region. Northern analysis with eight mitochondrial gene probes showed altered transcript pattern only for the coxI gene. Two transcripts of 2.0 and 2.4 kb, respectively, were detected in the CMS line. The novel 2.4 kb transcript was present in floral bud tissue but absent in the leaf tissue. In plants where male sterility broke down under high temperature during the later part of the growing season, the 2.4 kb coxI transcript was absent, which suggested its association with the CMS. The two coxI genes from the CMS line showed two amino acid changes in the coding region. The novel coxI gene showed unique repeats in the 5' region suggesting recombination of mitochondrial genomes of the two species. The possible role of the duplicated coxI gene in causing male sterility is discussed.
Subject(s)
Base Sequence , Brassica/genetics , Cyclooxygenase 1/genetics , Cytoplasm/genetics , DNA, Mitochondrial/analysis , Flowers/genetics , Gene Duplication , Gene Expression , Genome, Plant , Hybrid Cells/metabolism , Molecular Sequence Data , Mustard Plant/genetics , Plant Infertility/genetics , RNA/analysis , Random Amplified Polymorphic DNA Technique , Sequence Homology, Nucleic AcidABSTRACT
Complications occurring in 2,400 treadmill tests are reported, out of which 2107 (87.8%) were on males and 292 (12.2%) on females. The total number of patients with complications was 29 (1.2%) and the types were: acute MI in 2, ventricular tachycardia (sustained) in 3, nonsustained in 7 with ventricular couplets in 3 patients, atrial tachyarrhythmias in four of AVNRT in 2, EAT in 2, SVT with abberancy in 1, SVT (undifferentiated) in 3, bradyarrhythmias in form of sinus bradycardia in 4, hypotension in 4 and AV block in 2. There were no deaths. Only 8 (0.33%) patients required treatment-2 each of acute MI, sustained VT, AVNRT and hypotension, though only 4 of them were hospitalised (2 each of acute MI and VT) and subsequently discharged. Highest incidence of complications was seen in post MI patients (2.01%) followed by those with typical anginal pain (1.9%) and they had more serious problems in form of MI and sustained VT. 55.17% patients with complications were positive for provocative ischaemia as compared to 22.75% positivity in the total. Although 14 (38.2%) patients developed complications at workload of 4-6 METS but 2 achieved load of 10 METS also. Complications were seen mostly during exercise and also during recovery only.
Subject(s)
Arrhythmias, Cardiac/epidemiology , Coronary Disease/diagnosis , Exercise Test/adverse effects , Female , Humans , Incidence , Male , Middle Aged , Myocardial Infarction/epidemiology , Risk FactorsABSTRACT
Plasma lipoproteins in 30 patients of cortical infarction and 20 patients of lacunar infarction were estimated to study the relation of plasma lipids to the risk for ischaemic stroke by comparing clinical and biochemical characteristics of survivors. No significant differences were observed in the concentrations of total cholesterol, triglycerides, low density lipoproteins, very low density lipoproteins in both the groups. However patients with lacunar infarction had higher concentrations of high density lipoproteins as compared to patients with cortical infarction. These data suggest that previously demonstrated differences in HDL concentration between patients with ischaemic stroke and control subjects without stroke may be true for patients with cortical infarction but not for patients of lacunar infarction.
Subject(s)
Adult , Aged , Cerebral Infarction/blood , Female , Humans , Intracranial Arteriosclerosis/blood , Lipoproteins/blood , Lipoproteins, HDL/blood , Male , Middle Aged , Prospective Studies , Risk FactorsABSTRACT
A total of 45 cases of Aluminium phosphide poisoning were studied from December 1989 to November 1990. Silver nitrate (AgNO3) test was positive in gastric aspirate in all cases (100%) who took fresh tablets and negative in those who took exposed tablets. The test was also positive in breath but had a lower sensitivity. Majority of patients revealed ECG abnormalities.